Purification of Cyclospora cayetanensis oocysts obtained from human stool specimens for whole genome sequencing
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چکیده
منابع مشابه
Draft Genome Sequences from Cyclospora cayetanensis Oocysts Purified from a Human Stool Sample
The parasite Cyclospora cayetanensis causes foodborne diarrheal illness. Here, we report draft genome sequences obtained from C. cayetanensis oocysts purified from a human stool sample. The genome assembly consists of 865 contigs with a total length of 44,563,857 bases. These sequences can facilitate the development of subtyping tools to aid outbreak investigations.
متن کاملDetection of Cyclospora cayetanensis oocysts in human fecal specimens by flow cytometry.
A diagnosis of cyclosporiasis typically involves stool examinations for the presence of Cyclospora oocysts by means of microscopy. In recent years, flow cytometry has been gaining in popularity as a novel method of detecting pathogens in environmental and clinical samples. The present study is an evaluation of a flow cytometric method for the detection and enumeration of Cyclospora oocysts in h...
متن کاملHuman Challenge Pilot Study with Cyclospora cayetanensis
We describe a pilot study that attempted to infect human volunteers with Cyclospora cayetanensis. Seven healthy volunteers ingested an inoculum of Cyclospora oocysts (approximately 200-49,000 oocysts). The volunteers did not experience symptoms of gastroenteritis, and no oocysts were detected in any stool samples during the 16 weeks volunteers were monitored.
متن کاملEmerging pathogen Cyclospora cayetanensis infection in Nepal.
Cylospora cayetanensis, an emerging parasitic pathogen of human is being increasingly recognized throughout the world, however the means of transmission and the possibility of a reservoir host remain an enigma. A longitudinal study on cyclosporiasis in different parts of Nepal was carried out from April, 1995 until November, 2000. Fecal specimens were collected from symptomatic and asymptomatic...
متن کاملMultilocus Sequence Typing Tool for Cyclospora cayetanensis
Because the lack of typing tools for Cyclospora cayetanensis has hampered outbreak investigations, we sequenced its genome and developed a genotyping tool. We observed 2 to 10 geographically segregated sequence types at each of 5 selected loci. This new tool could be useful for case linkage and infection/contamination source tracking.
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ژورنال
عنوان ژورنال: Gut Pathogens
سال: 2018
ISSN: 1757-4749
DOI: 10.1186/s13099-018-0272-7